2.4.2.14	-	
2.4.2.14	amplified from seedling cDNA library to encode 11-amino acid autoprocessing propeptide, N-terminal cysteine for an active enzyme and deleted chloroplast transit peptide, in pCR2.1TOPO for sequencing, in pET26b for expression in Escherichia coli BL21(DE3)	
2.4.2.14	expressed in Escherichia coli BL21 cells	
2.4.2.14	expression in Escherichia coli	
2.4.2.14	expression of wild-type and K338Q/P422W mutant enzyme in amidophosphoribosyltransferase deficient CHO ade -A cells and in transgenic mice	
2.4.2.14	expression of wild-type, Y74A, Y258A, Y258F, K326Q, Y329A, G331I, N351A and Y465A mutant enzyme in Escherichia coli	
2.4.2.14	overexpression of wild-type and P410W mutant enzyme in Escherichia coli	
2.4.2.14	used in gene replacment and complementation experiments	
2.4.2.14	wildtype, mutant I198V and variants 1-04 and 2-02 of mutant I198V in pCA24N for expression as N-terminal hexa-His-tagged, C-terminal GFP-tagged proteins in Escherichia coli JMB9 DELTA TrpF (auxotrophic for tryptophan) and/or Escherichia coli KK8(pDM), mutant I198V in pCDF-1b for error-prone PCR based mutagenesis yielding variant 1-04