2.4.1.7	cloning from genetic library, complementation of growth deficient Escherichia coli strain JM109, DNA and amino acid sequence determination and analysis, expression in Escherichia coli	
2.4.1.7	DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B	
2.4.1.7	DNA and amino acid sequence determination and analysis, phylogenetic tree	
2.4.1.7	expressed in Acetobacter strain G7, enhanced cellulose production in transformed cells	
2.4.1.7	expressed in Escherichia coli BL21 (DE3). To enhance the soluble expression of the enzyme, several chaperones plasmids such as pG-KJE8 (DnaK-DnaJ-GrpE-GroES-GroEL), pGro7 (GroES-GroEL), pKJE7 (DnaK-DnaJ-GrpE), and pG-TF2 (GroES-GroELTig) are coexpressed and their coexpression conditions are optimized	
2.4.1.7	expression in Escherichia coli	
2.4.1.7	expression in Escherichia coli BL-21 Star on large scale	
2.4.1.7	expression in Escherichia coli BL21	
2.4.1.7	expression in Escherichia coli BL21. Expression conditions are optimized. The highest expression efficiency is obtained at an initial cell density of OD600 0.5, with 0.05 mM isopropyl-beta-D-thiogalactoside, followed by shaking at 180 rpm and incubation at 30°C for 15 h	
2.4.1.7	expression in Escherichia coli strain JM109