2.4.1.229 - 639557 2.4.1.229 cloning of a hybrid Gnt1, the coding sequence is generated from the 16 amino acid exon 1 of Dictyostelium discoideum Gnt1 and exon 2 of Dictyostelium purpureum Gnt1, and expression in Escherichia coli as His-tagged protein 721632 2.4.1.229 gene GnT51, DNA sequence determination and analysis, functional expression of wild-type and mutants as fusion proteins with CBD-intein, in Escherichia coli strain ER2566, autocleavage of tag moiety 639555