2.4.1.226 CSGlcAT/ChSy-3 quantitative tissue expression analysis by realtime RT-PCR, overview 723586 2.4.1.226 DNA and amino acid sequence determination and analysis, expression of putative catalytic domains of CSGlcAT-II and CSS2 in COS-7 cells as FLAG-tagged soluble proteins, which possesses glucuronyltransferase and N-acetylgalactosaminyltransferase activities as the wild-type enzyme 659234 2.4.1.226 enzyme domain containing beta-3-glycosyltransferase motifs, expressed in COS-7 cells ATCCCRL 1651 488969 2.4.1.226 expressed in COS-1 cells 690806 2.4.1.226 gene acbD, encoded within the presumed capsule biosynthesis gene cluster, functional recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein 758683 2.4.1.226 gene ChSy-1, genotyping in healthy persons and multiple sclerosis patients, recombinant expression of wild-type and mutant enzymes in COS-1 cells 736890 2.4.1.226 gene CHSY1, quantitative RT-PCR enzyme expressiona analysis 759875 2.4.1.226 gene chsy1, real-time PCR enzyme expression analysis 735915 2.4.1.226 gene CHSY1, soluble forms of wild-type and mutant ChSy-1 is heterologously expressed in COS-1 cells, the recombinant enzymes are secreted to the medium, quantitative real-time RT-PCR enzyme expression analysis 735696 2.4.1.226 genetic organization, coexpression of enzyme and chondroitin polymerizing factor as fusion protein in COS-1 cells, secretion into the cell culture medium 659218