2.4.1.109 - 2.4.1.109 chromosome mapping of PMT1 2.4.1.109 cloning and overexpression of PMT2 in yeast strain GFUII-4B, showing no alteration of enzyme activity, and co-overexpression with PMT1 in yeast strain TF1.8, leading to 3fold increase in enzyme activity in vitro, thus PMT1 and 2 function as a complex 2.4.1.109 DNA sequence determination 2.4.1.109 DNA sequence determination, chromosome mapping 2.4.1.109 expressed in Escherichia coli OrigamiTM (DE 3) cells 2.4.1.109 expressed in HEK-293T cells 2.4.1.109 expression in Saccharomyces cerevisiae 2.4.1.109 expression of Pmt4 in a Saccharomyces cerevisiae pmt1pmt4 mutant strain functionally complements the deficient Pmt activity 2.4.1.109 gene PAS_chr2-1_0212, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha