2.3.2.24 expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag 725413 2.3.2.24 expression in Escherichia coli 734838 2.3.2.24 gene UBE2O, cloned from liver, DNA and amino acid sequence determination and comparisons 759177 2.3.2.24 gene UBE2O, recombinant expression of Strep-tagged UBE2O in HEK-293 cells, coexpression with FLAG-tagged BMAL1 transcription factor 759488 2.3.2.24 recombinant expression of diversely tagged wild-type and mutant UCP enzymes in HEK-293T cells, the activity of the recombinant mutant enzymes varies dependent on the fusion tag, overview 760018