2.3.1.B25	DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis	722034	
2.3.1.B25	gene pksE, DNA and amino acid sequence determination and analysis	757727	
2.3.1.B25	recombinant expression of the gene encoding wild-type enzyme OKS in Aspergillus nidulans strain NID2252 controlled by the Aspergillus nidulans gpdA promoter as a single copy. Construction of a strain co-expressing OKS with ZhuI, which encodes the cyclases that catalyzes formation of the C7-C12 first ring closure. This strain shows 2.5, 2.2, and 2.1fold increases in flavokermesic acid, SEK4, and dehydro-SEK4 levels, respectively, compared to expression of OKS alone. This increase is 60% higher than what is observed with the strains expressing OKS in combination with either ZhuI (UniProt ID Q9F6D3) or ZhuJ (UniProt ID Q9F6D2), respectively, indicating that the cyclase and aromatase, in an additive manner, are able to increase the flux in the artificial de novo pathway towards the desired product, flavokermesic acid	758420	
2.3.1.B25	transient functional recombinant expression in Nicotiana benthamiana, coexpression with genes StZhuI and StZhuJ from Streptomyces sp., and DcUGT2 from Dactylopius coccus	756452