2.3.1.74 - 486402, 487453, 487476, 487479, 487482, 487483, 487484, 673859, 673860, 689725 2.3.1.74 an octaploid (Fragaria x ananassa cv. Calypso) genotype of strawberry is transformed with an antisense chalcone synthase (CHS) gene construct using a ripening related CHS cDNA from Fragaria x ananassa cv. Elsanta under the control of the constitutive CaMV 35S promoter via Agrobacterium tumefaciens. Out of 25 transgenic lines, nine lines showed a reduction in CHS mRNA accumulation of more than 50% as compared to the untransformed cv. Calypso control 674119 2.3.1.74 expressed in Arabidopsis thaliana mesophyll protoplasts 755796 2.3.1.74 expressed in Escherichia coli 676378 2.3.1.74 expressed in Escherichia coli BL21 (DE3) pLysS cells 757913 2.3.1.74 expressed in Escherichia coli BL21 cells 757489, 757756 2.3.1.74 expressed in Escherichia coli BL21(DE3) cells 757784, 758151 2.3.1.74 expressed in Escherichia coli BL21-Rosetta (DE3) cells 756731 2.3.1.74 expressed in Escherichia coli DH5alpha cells 756166, 757938 2.3.1.74 expressed in Escherichia coli Rosetta-gami (DE3) and BL21-Rosetta (DE3) cells 757487