2.3.1.172	expression of wild-type and mutant Ss5MaT1 in Escherichia coli	
2.3.1.172	from petals, expression in Escherichia coli JM109, sequencing	
2.3.1.172	into the entry vector pDONR207, the entry clone is used to transfer the open reading frame into the expression plasmid pH9GW for expression of the protein in Escherichia coli Bl21DE3 cells