2.1.1.218	expression in Escherichia coli	
2.1.1.218	gene TK0422, sequence comparisons, recombinant expression of N-terminally His6-tagged protein in Escherichia coli	
2.1.1.218	recombinant expression of His-tagged full-length wild-type enzyme, of truncated enzyme SaTrm10 1-249, and of enzyme mutants in Escherichia coli strain Rosetta (DE3)pLysS, recombinant expression of nontagged wild-type enzyme