1.3.2.3	-	
1.3.2.3	cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview	
1.3.2.3	expressed in Escherichia coli BL21(DE3) cells	
1.3.2.3	expressed in tobacco plants by Agrobacterium-mediated transformation under CaMV 35S constitutive promoter	
1.3.2.3	expression in Escherichia coli	
1.3.2.3	expression in Saccharomyces cerevisiae	
1.3.2.3	for expression in Escherichia coli BL21DE3 cells	
1.3.2.3	gene GDH-A1, from the nulli-tetrasomic lines of CS, chromosome 5A, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings	
1.3.2.3	gene GDH-B1, from the nulli-tetrasomic lines of CS, chromosome 5B, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings	
1.3.2.3	gene GDH-D1, from the nulli-tetrasomic lines of CS, chromosome 5D, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings