1.17.9.1 expressed in Escherichia coli 1.17.9.1 expressed in Escherichia coli as a His-tagged fusion protein 1.17.9.1 expressed in Escherichia coli BL21(DE3) 1.17.9.1 expressed in Escherichia coli BL21(DE3) cells 1.17.9.1 expressed in Escherichia coli Top10 cells 1.17.9.1 expression in Escherichia coli JM109 and Pseudomonas putida RA4007 1.17.9.1 expression of the flavoprotein subunit with non-covalently attached FAD in Escherichia coli 1.17.9.1 overexpression of the flavoprotein in Escherichia coli 1.17.9.1 the 9200 Da c-type cytochrome subunit from Pseudomonas putida NCIMB 9869 is overexpressed in recombinant form in Pseudomonas aeruginosa PAO1-LAC. Efforts to produce the cytochrome in Escherichia coli using a pET vector, with or without its signal peptide, are unsuccessful, yielding relatively low levels of the protein 1.17.9.1 the pchACXF operon encodes p-hydroxybenzaldehyde dehydrogenase and PCMH, mapping of the transcriptional start sites of the pch operons, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis, gene pchF expression in Escherichia coli strain DH5alpha