1.14.99.29 - 676855, 686708 1.14.99.29 construction of a polycistronic vector encoding eIF5A, DHS and DOHH, expression in Escherichia coli strain BL21(DE3), overview. Effects of time and temperature on the expression of recombinant proteins and hypusine modification, DOHH effectively hydroxylates all the eIF5A(Dhp), a deoxyhypusine-containing intermediate, formed 716821 1.14.99.29 DNA and amino acid sequence determination and analysis, different clones, sequence comparisons, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 689862 1.14.99.29 expressed in Escherichia coli BL21 cells 744002 1.14.99.29 expressed in Escherichia coli BL21(DE3) cells 746515 1.14.99.29 expressed in Escherichia coli BL21(DE3)pLysS cells 745386 1.14.99.29 expressed in Escherichia coli BL21-CodonPlus (DE3) cells 744989 1.14.99.29 expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 674901 1.14.99.29 for expression in Escherichia coli cells 700997 1.14.99.29 gene dohh 713727