1.14.19.31	DNA and amino acid sequence determination and analysis	
1.14.19.31	DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, recombinant functional expression in Saccharomyces cerevisiae strain HH3	
1.14.19.31	expression in MCF-7 human breast cancer cells. MCF-7 cells stably expressing FADS1 and FADS2	
1.14.19.31	expression in yeast and in Arabidopsis	
1.14.19.31	gene CrDELTA4FAD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged enzyme in Chlamydomonas reinhardtii chloroplasts, recombinant expression of the His-tagged cytochrome b5 domain (resdues 48-147) in Escherichia coli strain BL21(DE3)	
1.14.19.31	gene Egd4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme	
1.14.19.31	gene Old4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme, GFP-Old4p does not form aggregates in yeast cells and localizes to the endoplasmic reticulum	
1.14.19.31	gene TpdesK, encoded on chromosome 5, the gene has a single intron, phylogenetic analysis and tree, recombinant expression in Saccharomyces cerevisiae strain W303A1	
1.14.19.31	heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid