1.1.2.8	-	
1.1.2.8	a plasmid construct is used to express PQQ-DH9. DH9. The expression host is a derivative Gluconobacter sp. strain of CHM43, which lacks the genes for PQQ-dependent glycerol dehydrogenase and the membrane-bound alcohol dehydrogenase and consequently has minimal ability to oxidize primary and secondary alcohols. The membranes of the transformant exhibited considerable d-arabitol dehydrogenase activity, whereas the reference strain did not, even if it had PQQ-DH9-encoding genes in the chromosome and harbored the empty vector. This suggests that PQQ-DH9 is not expressed in the genome	
1.1.2.8	Acetobacter pasteurianus JSTS subunits I (adhA) and II (adhB) of the enzyme (PQQ-ADH) are amplified by PCR, subcloned into a vector, and transformed back into Acetobacter pasteurianus JST-S. The acetic acid production is improved by the engineered strain (61.42 g/l) while the residual ethanol content (4.18 g/l) is decreased	
1.1.2.8	C-terminally His-tagged wild-type and mutant enzyme forms are expressed in Escherichia coli BL21(DE3)	
1.1.2.8	gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs	
1.1.2.8	gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs	
1.1.2.8	gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs	
1.1.2.8	gene exaF, or exaA, DNA and amino acid sequence determination and analysis, functional recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain TOP10	
1.1.2.8	sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis