1.1.1.219 allele of DFR associated with red color, under the control of a doubled CaMV 35S promoter and tobacco etch virus translational enhancer introduced into the potato cultivar Prince Hairy (genotype dddd rrrr P-), which has white tubers and pale blue flowers 1.1.1.219 DFR2, DFR3 and DFR5 were expressed in Escherichia coli 1.1.1.219 enzyme expression analysis under UV-light irradiation, overview 1.1.1.219 Escherichia coli strains transformed with plasmid pTrcHis2-DFR to only express DFR. Escherichia coli strains harboring plasmid pET-DFR-LAR expressing DFR and leucoanthocyanidin reductase 1.1.1.219 exprerssion in Escherichia coli, Nicotiana benthamiana, and onion 1.1.1.219 expression in Escherichia coli 1.1.1.219 expression in Escherichia coli JM109 1.1.1.219 expression in Nicotiana tabacum 1.1.1.219 expression in Saccharomyces cerevisiae 1.1.1.219 functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity