1.1.1.202 a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli 747304 1.1.1.202 all genes of the dha regulon, including the gene for 1,3-propanediol oxidoreductase of Klebsiella pneumoniae mobilized by the plasmid RP4:mini Mu and transferred to Escherichia coli 389493 1.1.1.202 expressed in Escherichia coli BL21 cells 760717 1.1.1.202 expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli 667270 1.1.1.202 expression in Escherichia coli 389495, 710747, 721395, 725951 1.1.1.202 gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) 670216 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons 688171 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain JM109 739886 1.1.1.202 gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in YqhD-deficient Escherichia coli strain BL21(DE3) 740216 1.1.1.202 gene dhaT, expression of the N-terminally His-tagged in Escherichia coli strain BL21(DE3) 684171