1.1.1.12 derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells 696988 1.1.1.12 expression in Escherichia coli 669541, 684627, 711121 1.1.1.12 gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 740145 1.1.1.12 gene lad1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 739939 1.1.1.12 gene ladA 721913, 740772 1.1.1.12 gene ladA, DNA and amino acid sequence determination and analysis 740218 1.1.1.12 gene ladA, DNA and amino acid sequence determination and analysis, construction of strain 3M-43/pyrA, a pyrA mutant of strain 3M-43, in which an intact copy of ladA is inserted under control of the Aspergillus nidulans gpdA promoter. Complementation of the 3M-43 pyrA strain with pGpd:ladA 740218 1.1.1.12 gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 738728 1.1.1.12 gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 761551 1.1.1.12 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 762388