4.6.1.12 - 654130 4.6.1.12 a 339 bp core fragment and full-length DNA of MECPS amplified and subcloned into pGEM T-easy vector. Escherichia coli harboring both of the plasmids, pAC-BETA and pTrc or pTrcTmMECPS or pTrcAtMECPS 709487 4.6.1.12 amplification product digested with BamHI and PstI and ligated into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue 708124 4.6.1.12 cloned into pET17b for expression of the native polypeptide in Escherichia coli strain BL21 657313 4.6.1.12 expressed in Escherichia coli 691382 4.6.1.12 expressed in Escherichia coli strain BL21 (DE3) 709435 4.6.1.12 expressed in Escherichia coli strain EB370 730357 4.6.1.12 expression in Escherichia coli 655417 4.6.1.12 expression in Escherichia coli strain NMW26 and Arabidopsis thaliana 666594 4.6.1.12 final amplification product digested with the restriction endonucleases BamHI and SalI and cloned into plasmid pQE30, expressed in Escherichia coli strain XL1-Blue 708124