3.5.1.54 gene atzF, DNA and amino acid sequence determination and analysis, recombinant expression of functional His-tagged enzyme in Escherichia coli strain BL21 (lambdaDE3) 3.5.1.54 gene atzF, expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 3.5.1.54 gene atzF, qualitative and quantitative RT-PCR enzyme expression analysis 3.5.1.54 gene atzF, sequence comparisons, cloning of atzF and the truncated gene encoding the amidase (N-terminal) domain of AtzF (atzF467), recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 3.5.1.54 gene CGDNIH1, expression of N-terminally His8-tagged wild-type and mutant enzymes in Escherichia coli strain HMS174 (DE3) 3.5.1.54 gene KLLA0_E08119g, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)Star 3.5.1.54 gene trzF, overexpression of His-tagged enzyme in presence of a chaperone in Escherichia coli strain BL21(DE3) 3.5.1.54 overexpression in Escherichia coli strain BL21(DE3) 3.5.1.54 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 3.5.1.54 recombinant expression of wild-type and mutant His-tagged SeMet-substituted enzymes in Escherichia coli strain BL21 Star(DE3)