2.4.1.90 cloned into pMAL-c4X vector and expressed in Escherichia coli Origami B (DE3) cells as an N-maltose binding protein (MBP)-tagged and C-His6-tagged fusion protein 719274 2.4.1.90 cloning and sequencing of cDNA 489501 2.4.1.90 cloning and sequencing of the full-length cDNA 489497 2.4.1.90 comparison of sequences of enzyme from placenta and HeLa cells 489502 2.4.1.90 DNA and amino acid sequence determination and analysis, promoter activity analysis 687455 2.4.1.90 DNA fragments coding the catalytic domain (Cd7) sequence (residues 71-322) and the C-terminal 11-amino acid deletion (Cd7DELTAC) sequence (residues 71-311) are cloned into a pET23a vector and expressed in Escherichia coli 719863 2.4.1.90 enzymatically active soluble N-deglycosylated enzyme form 489554 2.4.1.90 enzyme fused to protein A is expressed as a soluble form in COS-7 cells 489543 2.4.1.90 expressed in a silkworm-baculovirus expression vector system 757639 2.4.1.90 expressed in Escherichia coli BL21(DE3) cells 756991, 758173