2.3.1.158 DNA sequence determination and analysis, functional overexpression in Arabidopsis thaliana roots and leaves 660241 2.3.1.158 expressed in Pichia pastoris 755830 2.3.1.158 expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter 685341 2.3.1.158 expression in Saccharomyces cerevisiae 736422 2.3.1.158 expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves 756132 2.3.1.158 expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves. CsPDAT1-A shows a higher preference for alpha-linolenic acid (18:3 omega-3) 756132 2.3.1.158 gene encoding PDAT, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of full-length enzyme and of truncated PDAT lacking the transmembrane domain in Pichia pastoris 720694 2.3.1.158 gene LRO1, DNA and amino acid sequence determination and analysis 757544 2.3.1.158 gene MiPDAT, DNA and amino acid sequence determination and analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Nicotiana benthamiana leaves via transfection method with Agrobacterium tumefaciens, subcloning in Escherichia coli strain DH5alpha, recombinant expression of PDAT in TAG-deficient Saccharomyces cerevisiae mutant strain H1246, complementation and lipid analysis, overview 758363 2.3.1.158 gene pdat or YALI0E16797g, DNA and amino acid sequence determination and analysis. In vivo expression of DGAT1, DGAT2 and PDAT under the heterologous Yarrowia lipolytica YAT promoter results in 598%, 702% and 278% increases in total fatty acid % dry cell weight over the empty vector control 721119