2.3.1.12 - 348961, 348982, 393963, 486166, 486169, 486171, 486179, 486224, 486227, 486228, 486229, 486233, 486236, 486238, 486240 2.3.1.12 a recombinant fragment hL2S (containing the second lipoyl domain (L2), second hinge region, E1-binding domain (S) and third hinge region of hE2, residues 128–330) is overexpressed in Escherichia coli 686772 2.3.1.12 catalytic domain, residues 173-427 486225 2.3.1.12 expressed in Escherichia coli 672273, 690088 2.3.1.12 expressed in Escherichia coli AG1 cells 736459 2.3.1.12 expressed in Escherichia coli BL21(DE3) cells 725521, 736448 2.3.1.12 expression in Escherichia coli 720722 2.3.1.12 expression of subgene for lipoyl domain in Escherichia coli 486167 2.3.1.12 expression of the enzymes' L2 domain as GST-fusion protein 662151 2.3.1.12 functional overexpression of the enzyme E2 with a single hybrid lipoyl domain per subunit, i.e. 1-lip E2, in strain JRG1342 and BL21(DE3), overexpression of the isolated hybrid lipoyl domain in strain JM 101 663289