2.1.1.182 - 702903, 703844, 705774, 706715, 706787 2.1.1.182 cloning of ksgA from the 431DELTA99 KSGR mutant 704036 2.1.1.182 expression of a pGEX-KsgA fusion construct in Escherichia coli KSR7 705968 2.1.1.182 expression of the Chlamydia trachomatis L2 KsgA ortholog restored kasugamycin sensitivity to the Escherichia coli ksgA mutant 702903 2.1.1.182 expression of wild-type and mutant enzymes in Escherichia coli strain XL1-Blue 718932 2.1.1.182 full-length ksgA gene is cloned into the expression vector pET26b and overexpressed in Escherichia coli strain BL21Star 705141 2.1.1.182 KsgA engineered with an N-terminal polyhistidine tag is overexpressed in Escherichia coli cells 662620