2.1.1.179 - 710880, 710881, 712741 2.1.1.179 cloned in Streptomyces lividans. The sgm gene is expressed in Micromonospora melanosporea, where its own promoter is active, and also in Escherichia coli under the control of the lacZ promoter 704248 2.1.1.179 cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression 705988 2.1.1.179 expressed in Escherichia coli BL21(DE3) cells 733120 2.1.1.179 expression in Escherichia coli 702415, 704280 2.1.1.179 expression in Escherichia coli, Bacillus subtilis or Staphylococcus aureus 712038 2.1.1.179 gene fmrO, phylogenetic analysis, sequence comparisons 719353 2.1.1.179 gene grmA, phylogenetic analysis, sequence comparisons 719353 2.1.1.179 gene grmB, phylogenetic analysis, sequence comparisons 719353 2.1.1.179 gene kmr, phylogenetic analysis, sequence comparisons 719353