1.9.6.1 - 697968 1.9.6.1 cloning of napABC, quantitative PCR-based expression analysis 742645 1.9.6.1 expressed in Escherichia coli 698636 1.9.6.1 gene nap and nap gene cluster, genetic organization and sequence comparisons 742319 1.9.6.1 gene nap, expression analysis 742380 1.9.6.1 gene napA, enzyme NapA is encoded, along with its periplasmic di-heme c-type cytochrome redox partner NapB, in the seven gene nap operon, coexpression of NapA with His-tagged NapD activator in Escherichia coli strains MC4100 and BL21(DE3), recombinant expression of MTSL-labelled MalE-NapASP fusion mutant S4C/S24C in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain LCB2048 742486 1.9.6.1 structural genes, napA and napB, are cloned, and their nucleotide sequences is determined 698544