1.14.11.6 DNA and amino acid sequence determination and analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) 697315 1.14.11.6 expressed in Escherichia coli BL21(DE3)Gold 658220 1.14.11.6 expression in Escherichia coli, fusion protein with green fluorescent protein and with His-tag 689246 1.14.11.6 expression of either the wild type JBP2 or the mutant JBP2 proteins in insect stage trypanosomes, which do not contain the modified base J and determined the base J content of each cell line, mutations made within the key catalytic domain ablates the ability of JBP2 to initiate de novo base J synthesis 700501 1.14.11.6 JBP2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes, the latter contain 4 exchanges of conserved residues 700084 1.14.11.6 recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Codon Plus 745921