1.13.11.46 expressed in Escherichia coli 1.13.11.46 expression of C-terminally strep-tagged enzyme in Escherichia coli strain BL21(DE3) 1.13.11.46 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 1.13.11.46 recombinant expression of wild-type and mutant enzymes