7.6.2.8 gene ABCD4, stable recombinant expression of human ABCD4 in CHO cells, recombinant expression of N-terminally MBP-tagged enzyme, stable recombinant expression of wild-type and mutant HA-tagged ABCD4 in human hepatoma HuH7 cells and additional transient coexpression of wild-type and mutant GFP-tagged LMBRD1 752200 7.6.2.8 gene btuF, phylogenetic tree of BtuF, recombinant expression of His6-tagged BtuF in Escherichia coli strain XL1-Blue 749978 7.6.2.8 gene Tbd_2719 or btuM, recombinant expression of codon-optimized enzyme in Escherichia coli strain MC1061 and in Escherichia coli strain JW0154 (DELTAbtuF::KmR::DELTA FEC) lacking active BtuF and BtuC, overexpression of His-tagged enzyme and of EPEA-tagged enzyme 751670 7.6.2.8 genes btuC and btuD form a cluster, encoding permease protein btuC and ATP-binding protein btuD, forming the ABC transporter BtuCD, expression of wild-type and utant enzymes as His-tagged proteins 697950 7.6.2.8 genes btuC, btuD, and btuF, recombinant expression of wild-type and mutant BtuCD-F complex components 751661 7.6.2.8 phage M13mp8 or M13mp9 210326 7.6.2.8 recombinant expression of ECF-CbrT complements in an Escherichia coli strain lacking its endogenous vitamin B12 transporter (Escherichia coli strain DELTAFEC, with btuF and btuC also deleted , thus abolished endogenous Cbl-uptake, from parental strain JW0154). Recombinant ECF-CbrT supports cobalamin-dependent growth of an Escherichia coli deletion strain 750412 7.6.2.8 recombinant expression of enzyme complex BtuCD-F in Escherichia coli strain BL21 (DE3) Gold 752215 7.6.2.8 recombinant expression of N-terminally His10-tagged BtuCD in Escherichia coli, recombinant expression of wild-type and mutant His6-tagged BtuF variants in Escherichia coli 752221