6.5.1.3 - 1986, 650172, 653889, 653915, 674568 6.5.1.3 encoded by T4 gene 24.1, mutants expressed in Escherichia coli 652387 6.5.1.3 enzymatically active Leishmania tarentolae REL1 and REL2 and Trypanosoma brucei REL1 proteins from a Baculovirus expression system. The recombinant proteins can each integrate into an L-complex that has been depleted of the cognate ligase by RNAi. In the case of REL1, the integration of the recombinant enzyme functionally complements the REL1-depleted L-complex in an in vitro editing system 663245 6.5.1.3 expressed in Escherichia coli 650982 6.5.1.3 expressed in Escherichia coli as a His-tagged fusion protein 728788, 728789 6.5.1.3 expressed in Escherichia coli as a His10-tagged fusion protein 654015 6.5.1.3 expressed in Escherichia coli BL21(DE3) cells 749277, 749330 6.5.1.3 expressed in Sf9 insect cells 716670 6.5.1.3 expressed in Trypanosoma brucei lines as an epitope-tagged fusion protein, recombinant p48 also expressed in Escherichia coli 653869 6.5.1.3 expression in Escherichia coli 676215, 694452 6.5.1.3 expression in Escherichia coli BL21goldDE3 662636 6.5.1.3 overexpressed in Escherichia coli BL21 (DE3) 650653 6.5.1.3 RNA ligase gene is inserted into a pET23b expression vector with a C-terminal His6-tag. This vector is transformed into Escherichia coli strain BL21 662976 6.5.1.3 RnlA gene overexpressed in Escherichia coli BL21 653392 6.5.1.3 transformed into Escherichia coli BL21 (DE3) 652429 6.5.1.3 transformed into Escherichia coli BL21(DE3) 653679