6.3.4.15 - 1443, 713831, 714197, 714612, 727901 6.3.4.15 coding region of birA biotin-protein ligase gene cloned from genomic DNA by PCR, verfied by sequencing and expressed in mouse erythroleukemic cells 653596 6.3.4.15 codon-optimized expression in Escherichia coli 745716 6.3.4.15 construction and expression of the C-terminal 87 amino acids of biotin carboxyl carrier protein, i.e. BCCP-87. cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA- strain of Escherichia coli 691104 6.3.4.15 cross-species activity of BPLs permitts functional cloning of the cDNA for human BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli 691104 6.3.4.15 cross-species activity of BPLs permitts functional cloning of the cDNA for yeast BPL through genetic complementation with a conditional lethal birA? strain of Escherichia coli 691104 6.3.4.15 DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 663526 6.3.4.15 doxycycline-regulated expression of a secreted variant of Escherichia coli biotin ligase BirA with target proteins containing a 13-residue biotin acceptor peptide appended to their extracellular domain, co-expression with three different G-protein coupled receptors, i.e. protease-activated receptors 1 and 2, and the platelet ADP receptor, P2Y12, which become biotinylated, in CHO-K1 Tet-On cells on the cell surface, overview 694961 6.3.4.15 expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells 728734 6.3.4.15 expressed in Escherichia coli BL21 cells 728764 6.3.4.15 expressed in Escherichia coli DH5alpha cells 726763 6.3.4.15 expression as His6-tagged enzyme in strain BL21(DE3) 694319 6.3.4.15 expression in Escherichia coli 716690 6.3.4.15 expression in Escherichia coli BL21(DE3) 671759 6.3.4.15 expression in Escherichia coli strain JM109 695558 6.3.4.15 expression in Escherichia coli strains DH5alpha and BL21(DE3) 690542 6.3.4.15 expression of BirA from pET-based vector pHBA in Escherichia coli BL21 (lambdaDE3) 653788 6.3.4.15 expression of HCS in Spodoptera frugiperda Sf9 cells and in Hi5 insect cells using the baculovirus transfection system 702086 6.3.4.15 expression of His9-tagged BPL in Saccharomyces cerevisiae strain w303, functional complementation of the conditional lethal birA85 Escherichia coli strain BM4062 690627 6.3.4.15 gene birA, expression of His-tagged wild-type and mutant enzymes in strain JM109, expression of enzyme in strain BM4092 unbiotinylated form 666851 6.3.4.15 gene birA, functional expression of N-terminally and C-terminally multiply tagged enzyme in Saccharomyces cerevisiae, low level expression of the surface-displayed enzyme, expression of avitagged-BirA in Saccharomyces cerevisiae, overview 664436 6.3.4.15 gene Rv3279c, DNA and amino acid sequence determination and analysis, expression of C-terminally Strep-tagged BirA in Escherichia coli 690261 6.3.4.15 overexpression in Escherichia coli 692748, 724993 6.3.4.15 overexpression of the enzyme comprising residues 1-125 in Escherichia coli strain BL21 666059