6.1.1.5 - 458, 459 6.1.1.5 expressed in Escherichia coli 727946 6.1.1.5 expression in Escherichia coli 457 6.1.1.5 expression of His-tagged wild-type and mutants enzymes in Escherichia coli 675388 6.1.1.5 gene IARS2, mitochondrial genome-encoded enzyme, genotyping 745057 6.1.1.5 gene ileS, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 745362 6.1.1.5 gene ileS, phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta (DE3) 745362 6.1.1.5 gene ileS, quantitative real-time PCR expression analysis 715118 6.1.1.5 gene ileS, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 744324 6.1.1.5 gene ileS, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), and overexpression in Escherichia coli strain B834(DE3) as selenomethionine-labeled protein 745675 6.1.1.5 gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331 652415 6.1.1.5 genes ileS1 and ileS2, phylogenetic analysis 745362 6.1.1.5 genotyping of thiaisoleucine-resistant enzymes, expression of GFP-tagged cytoplasmic isozyme in erythrocytic stage parasite 716781 6.1.1.5 into the vector pET3D-FLAG for expression in Escherichia coli BL21DE3 cells 704642 6.1.1.5 overexpression of His-tagged IleRS in Escherichia coli strain BL21 (DE3) 715519 6.1.1.5 subcloning and expression of wild-type and mutant enzymes in Escherichia coli Top10 cells 746476 6.1.1.5 with the pCR2.1-TOPO TA cloning kit for sequencing 705210