5.5.1.14 expression of the full-length preprotein including the transit peptid in Escherichia coli, protein alone and as a C-terminal fusion to glutathione-S-transferase. Additionally, a partial cDNA sequence resembling the mature native protein was expressed alone as a potential pseudomature protein 5.5.1.14 expression of the full-length protein as a fusion protein with glutathione-S-transferase at the N-terminus in Escherichia coli XL1-Blue 5.5.1.14 genotyping of the Tos17 insertion mutant