5.3.99.4 - 5.3.99.4 expressed in Mus musculus mesenchymal stem cells 5.3.99.4 expression in alveolar and airway epithelial cells from Mus musculus 5.3.99.4 expression in Cos-1 cells 5.3.99.4 expression in COS-7 cells 5.3.99.4 expression in Escherichia coli 5.3.99.4 expression in HEK 293 cells 5.3.99.4 expression in Spodoptera frugiperda 21 cells 5.3.99.4 expression with C-terminal His-tag and hydrophilic aminoterminal segment MAKKTSS 5.3.99.4 for expression in Escherichia coli cells 5.3.99.4 from endothelilal cells 5.3.99.4 fusion protein linking cyclooxygenase COX-2 and prostacyclin synthase together, separated by a transmembrane domain of 10 or 22 residues 5.3.99.4 GFP-tagged cDNA containing the full-length rat prostacyclin synthase gene is inserted into an adenoviral DNA construct for in vitro gene transfer to murine and human mesenchymal stem cells 5.3.99.4 into the pcDNA3.1 vector for transfection of insulin-producing RINm5F cells 5.3.99.4 recombinant expression of C-terminally His4-tagged enzyme with the first 16 amino acid residues in the N-terminal transmembrane domain replaced with a hydrophilic sequence, MAKKTSS, in Escherichia coli strain BL21/(DE3)pLys 5.3.99.4 recombinant expression of C-terminally His4-tagged enzyme with the first 17 amino acid residues in the N-terminal transmembrane domain replaced with a hydrophilic sequence, MAKKTSS, in Escherichia coli strain BL21/(DE3)pLys 5.3.99.4 specific transgenic expression in mouse kidney using rat KSP-cadherin promoter