5.1.3.14 bifunctional enzyme EC 5.1.3.14/EC 2.7.1.60, expressed in COS7 cells 2279 5.1.3.14 cloning of the neuC gene into an intein expression vector to facilitate purification 651736 5.1.3.14 DNA sequencing of the GNE coding region of 64 symptomatic patients with autosomal recessive hereditary inclusion body myopathy, genotyping-phenotyping of patients from different ethnics, overview 703865 5.1.3.14 expression in Arabidopsis thaliana 689622 5.1.3.14 expression in Chinese Hamster Ovary (CHO) 673699 5.1.3.14 expression in Escherichia coli 674638, 728782 5.1.3.14 expression in Escherichia coli, C-terminal His-tag 692855 5.1.3.14 expression in insect cells 675445 5.1.3.14 expression in Insect Cells and supercompetent InvalphaF' Escherichia coli cells (Invitrogen) 672131 5.1.3.14 expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722 660988 5.1.3.14 expression of M712T mutant enzyme in Sf9 insect cells 661735 5.1.3.14 expression of wild-type and mutant enzymes (C13S, H132Q, D176V, D177C, V331A, I472T, G708S, A631V, V572L and A630T) in COS-7 cells 662186 5.1.3.14 functional expression in Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris or insect cells. In all cell types, the expressed enzyme displayes both epimerase and kinase activities. In Escherichia coli up to 2 mg protein/l cell culture is expressed in yeast cells only 0.4 mg/ml, in insect cells up to 100 mg/L. In all three cell systems, insoluble protein aggregates are also observed. Expression and purification of the UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in Sf-900 cells can yield the miligram amount of protein required for structural characterization of the enzyme. The easier expression in Escherichia coli and yeast provides sufficient quantities for enzymatic and kinetic characterization 663299 5.1.3.14 gene gneY, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons 748089 5.1.3.14 gene gneYZ, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons 748089 5.1.3.14 gene mnaA, genotyping and mutation identification 755205 5.1.3.14 gene sacA, encoded in the serogroup A cps operon, sequence comparisons, recombinant overexpression of C-terminally His6-tagged recombinant NmSacA in Escherichia coli strain BL21(DE3) 747462 5.1.3.14 gene sacA, recombinant expression of C-terminally His6-tagged enzyme NmSacA in Escherichia coli strain BL21(DE3) 747462 5.1.3.14 gene wecB, sequence comparisons, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) 749249 5.1.3.14 gene wecB, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 749249 5.1.3.14 high level overexpression of the active enzyme is established by using the baculovirus/Sf9 system 641739 5.1.3.14 overexpression in Escherichia coli 649142 5.1.3.14 overexpression in Escherichia coli BL21(DE3), wild-type enzyme and mutant enzymes D100N, E122Q and D131N 661102 5.1.3.14 proteome expression analysis in wild-type and GNE-deficient embryonic stem cells, overview. GNE overexpression in HEK cells 703905 5.1.3.14 splice variant hGNE2, expression in insect and mammalian cells. Splice variant hGNE3, expression in Escherichia coli 692536 5.1.3.14 splice variants mGNE1, mGNE2, mGNE3 692536 5.1.3.14 wild-type and mutant enzymes expressed in Escherichia coli 661247