5.1.1.21	expressed in Escherichia coli	
5.1.1.21	gene daar1, genotyping, untargeted metabolite profiling and genome-wide association analysis on 440 natural accessions of Arabidopsis thaliana to establish genetic linkages between metabolites and genes, recombinant expression of His-tagged enzyme DAAR1 in Escherichia coli strain Rosetta 2 pLysS. The affinity tag does not affect enzyme activity or stability	
5.1.1.21	recombinant expression of codon-optimized, His-tagged enzyme in Escherichia coli strain BL21(DE3)	
5.1.1.21	sequence comparisons, recombinant expression of His/ProS2-tagged wild-type and mutant enzymes