4.4.1.9 expressed in Arabidopsis thaliana and in Saccharomyces cerevisiae 4.4.1.9 expression in Escherichia coli 4.4.1.9 expression of PCAS-1 in Escherichia coli 4.4.1.9 expression of PCAS-2 in Escherichia coli 4.4.1.9 full-length MdCAS2 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) 4.4.1.9 full-length pMdCAS1 cDNA clones introduced into plasmid pMAL-c2X, expressed in Escherichia coli BL21 (DE3) 4.4.1.9 gene Tu-CAS , DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli 4.4.1.9 recombinant enzyme expression as N-terminally His-tagged, functional dimeric type and mutant enzyme proteins lacking the mitochondrial localization sequence