4.2.3.19 - 4.2.3.19 as a GST-fusion protein for expression in Escherichia coli 4.2.3.19 DNA and amino acid sequence determination of genes KS1-9, chromosomal localizations, gene KS9 is a pseudogene, phylogenetic analysis, overview 4.2.3.19 expressed in Escherichia coli as fusion protein with maltose-binding protein, enzyme exclusively shows EC 4.2.3.19 activity 4.2.3.19 expressed in Escherichia coli BL21 (DE3) and MG1655 cells 4.2.3.19 expressed in Escherichia coli BL21 cells 4.2.3.19 expressed in Escherichia coli BL21(DE3) cells 4.2.3.19 expressed in Escherichia coli BL21DE-C41 cells 4.2.3.19 expressed in Escherichia coli BL21DE3-C41 cells 4.2.3.19 expressed in Escherichia coli C41 (DE3) cells 4.2.3.19 expressed in Escherichia coli C41 cells 4.2.3.19 expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity 4.2.3.19 expressed in Escherichia coli JM109, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity 4.2.3.19 expressed in Escherichia coli M15(DE3) cells 4.2.3.19 expressed in Escherichia coli OverExpress C41 cells 4.2.3.19 expressed in Escherichia coli strain C41 4.2.3.19 expressed in Escherichia coli strain Top10F- 4.2.3.19 expressed in Escherichia coli Top10 cells 4.2.3.19 gene KS, phylogenetic tree, quantitative real-time PCR expression analysis, functional recombinant expression in Escherichia coli strain C41(DE3), GC-MS analysis of diterpene products from hexane extracts of recombinant Escherichia coli harboring the pSdGGeC and pSdKS vectors 4.2.3.19 gene KS1, a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655 4.2.3.19 gene PHYPA_009773, quantitative real-time PCR and semiquantitative RT-PCR enzyme expression analysis 4.2.3.19 gene PHYPA_009773, recombinant expression in Escherichia coli strain C41, transient expression of PpCPSKS with the suppressor of silencing P19 in leafs of 4-weeks-old Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL-1 mediated transformation 4.2.3.19 in Escherichia coli as glutathione-S-transferase fusion protein 4.2.3.19 into the pTD1 vector as a fusion of copalyl diphosphate synthase and ent-kaurene synthase for in vitro expression 4.2.3.19 isozyme determination and phylogenetic analysis and tree, sequence comparisons, overview. Synthetic RcKS(L) isozymes are truncated to remove the N-terminal plastid-directing transit peptide sequences, they are individually subcloned into compatible expression vectors and each coexpressed with either the geranylgeranyl phosphate synthase (GGPS), or the GGPS along with a CPS (EC 5.5.1.13) for recombinant expression in Escherichia coli 4.2.3.19 overexpression of CPS and KS in transgenic Arabidopsis thaliana plants, expression in Escherichia coli 4.2.3.19 recombinant expression of enzyme mutant H212A in Escherichia coli 4.2.3.19 recombinant expression of enzyme mutant H302A in Escherichia coli 4.2.3.19 recombinant expression of thioredoxin- and His-tagged wild-type and mutant enzymes with or without selenium-methionine labeling in Escherichia coli strain BL21trxB (DE3) 4.2.3.19 sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain C41(DE3) 4.2.3.19 sequence comparisons, recombinant expression of wild-type enzyme in Escherichia coli strain C41(DE3) 4.2.3.19 SmKS DNA and amino acid sequence determination and analysis of full-length enzyme from cDNA, sequence comparisons and phylogenetic analysis and tree, recombinant enzyme expression in Saccharomyces cerevisiae strain BY-T20