4.2.3.15	-	730124	
4.2.3.15	expressed in Escherichia coli	210753, 748002	
4.2.3.15	expression in Escherichia coli	649430, 661304, 705893	
4.2.3.15	gene TPS2, cloning from a cDNA library, sequence comparisons and phylogenetic analysis and tree, quantitative RT-PCR expression analysis, recombinant expression of His-tagged enzyme without after removing the N-terminal plastid targeting sequence in Escherichia coli strain BL21(DE3)	747873	
4.2.3.15	mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET200-D-TOPO harbouring the respective AcTPS2 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli.	693257