4.2.2.19	expression in Escherichia coli as N-terminally His-tagged enzyme, 2 different expression systems	
4.2.2.19	gene cslB, expression in Escherichia coli without the N-terminal signal sequence, different expression plasmids constructed, expression in soluble and insoluble form	
4.2.2.19	overexpression of enzyme, residues 25-506, in Flavobacterium heparinum cells