4.1.99.5 affinity-tagged version expressed in Escherichia coli 715300 4.1.99.5 cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3) 727215 4.1.99.5 expressed in Escherichia coli 713511 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli 726957 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 726548 4.1.99.5 gene Npun_R1711, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 727690 4.1.99.5 gene PMT1231, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain BW25113(DE3) DELTA13 747353 4.1.99.5 gene PMT_1231, recombinant expression in Escherichia coli 747074 4.1.99.5 gene Synpcc7942_1593, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha 747351 4.1.99.5 gene Synpcc7942_1593, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 749212 4.1.99.5 His-tagged version expressed in Escherichia coli 715293 4.1.99.5 overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) 727001 4.1.99.5 recombinant expression in Escherichia coli 726552 4.1.99.5 recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 748564 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 748564 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 748564 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 748564 4.1.99.5 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS 749051 4.1.99.5 recombinant expression of mutants V41Y and A134F 727312 4.1.99.5 recombinant overexpression in Escherichia coli 715293 4.1.99.5 sequence comparisons 746976