4.1.99.5 affinity-tagged version expressed in Escherichia coli 4.1.99.5 cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3) 4.1.99.5 expressed in Escherichia coli 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 4.1.99.5 gene Npun_R1711, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 4.1.99.5 gene PMT1231, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain BW25113(DE3) DELTA13 4.1.99.5 gene PMT_1231, recombinant expression in Escherichia coli 4.1.99.5 gene Synpcc7942_1593, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha 4.1.99.5 gene Synpcc7942_1593, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 4.1.99.5 His-tagged version expressed in Escherichia coli 4.1.99.5 overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) 4.1.99.5 recombinant expression in Escherichia coli 4.1.99.5 recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis 4.1.99.5 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS 4.1.99.5 recombinant expression of mutants V41Y and A134F 4.1.99.5 recombinant overexpression in Escherichia coli 4.1.99.5 sequence comparisons