4.1.3.4 - 4.1.3.4 cloning of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein mutant G2A in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein in Escherichia coli and Pichia pastoris, in insoluble form 4.1.3.4 expressed as His-tagged recombinant protein in Escherichia coli pTrcAtuE cells 4.1.3.4 expressed in Escherichia coli 4.1.3.4 expressed in Escherichia coli BL21 cells 4.1.3.4 expressed in Escherichia coli DH5alpha cells 4.1.3.4 expressed in Escherichia coli JM105 cells 4.1.3.4 expression in Escherichia coli 4.1.3.4 gene hcl1 4.1.3.4 His-tagged version 4.1.3.4 His-tagged version expressed in Escherichia coli BL21(DE3) 4.1.3.4 mitochondrial and peroxisomal enzyme 4.1.3.4 nucleotide sequence 4.1.3.4 nucleotide sequence, identification of a 2-base pair deletion in S69 codon of enzyme-deficient patients 4.1.3.4 overexpression of wild-type enzyme and synthetic C323 mutant 4.1.3.4 sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase wild-type in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase in Escherichia coli and Pichia pastoris, in active form