4.1.2.52 HpaI wild type and R70A mutant expressed in Escherichia coli BL21(lambda DE3) under the control of the T7 promoter from expression plasmid pT7-7 4.1.2.52 HpcH gene expressed in Escherichia coli strain B834 (DE3) 4.1.2.52 mutant HpaI enzymes expressed in Escherichia coli Bl21(lambda DE3) cells