4.1.1.111 expressed in Escherichia coli BL21(DE3) cells 4.1.1.111 expression in Escherichioa coli 4.1.1.111 proteins ahbA and ahbB are cloned into distinct plasmids and expressed separately in Escherichia coli. When expressed individually the encoded proteins are highly unstable and can only be purified at very low concentrations due to their tendency to precipitate