3.5.99.7	-	34589, 648266, 648267, 667784	
3.5.99.7	ACC-deaminase encoding gene acdS, and a regulator gene lrp, i.e. acdR	719206	
3.5.99.7	An ACC deaminase minus mutant (AcdS) is constructed by the insertion of a tetracycline resistance gene into the coding region of the bacterial ACC deaminase gene.	692374	
3.5.99.7	cloning and genotype of Pseudomonas brassicacearum strains, overview	688071	
3.5.99.7	DNA and amino acid sequence determination and analysis, phylogenetic analysis	687194	
3.5.99.7	DNA and amino acid sequence determination and analysis, sequence compariosn and phylogenetic analysis	720283	
3.5.99.7	enzyme expression in Agrobacterium tumefaciens strain C58 leading to increased gene transfer of the recombinant bacterium in transfected plant cells, overview	684575	
3.5.99.7	expressed in Escherichia coli DH5alpha cells	713327	
3.5.99.7	expressed in Escherichia coli JM109 cells	712035	
3.5.99.7	expressed in Escherichia coli Rosetta(DE3) cells	733883	
3.5.99.7	expressed in Escherichia coli strain DH5alpha	711806	
3.5.99.7	expressed in Escherichia coli strain DH5alphaPRO	713222	
3.5.99.7	expression in Escherichia coli	648261	
3.5.99.7	expression in Escherichia coli and in delayed ripening tomato	648259	
3.5.99.7	gene acdS, DNA and amino acid sequence determination and analysis, gene mapping and phylogenetic analysis, fate of the acdS/acdR locus during phenotypic variation in Azospirillum lipoferum 4B	686779	
3.5.99.7	gene acdS, DNA and amino acid sequence determination and analysis, genotyping	758082	
3.5.99.7	gene acdS, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis	746770	
3.5.99.7	gene acdS, DNA and amino acid sequence determination and analysis, genotyping, phylogenetic analysis and tree	758498	
3.5.99.7	gene acdS, DNA and amino acid sequence determination, phylogenetic analysis using 16S RNA genetic sequence	719749	
3.5.99.7	gene acdS, functional recombinant expression of the exogenous 1-aminocyclopropane-1-carboxylate deaminase gene from Pseudomonas putida in the psychrotolerant bacteria, acdS negative Flavobacterium sp. strain OR306 and Pseudomonas frederiksbergensis strain OS211	758070	
3.5.99.7	gene acdS, phylogenetic analysis	755818	
3.5.99.7	gene acdS, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta	757627	
3.5.99.7	gene acdS, recombinant expression of His6-tagged ACCD in Escherichia coli strain BL21(DE3)	757901	
3.5.99.7	gene acdS, recombinant overexpression in Sinorhizobium meliloti strain CCNWSX0020 (ACCC 19736), a Cu-resistant strain isolated from the root nodules of Medicago lupulina plants growing in lead-zinc mine tailings in China, quantitative real-time PCR analysis	758081	
3.5.99.7	gene acdS, RT-PCR enzyme expression analysis	757612	
3.5.99.7	gene acdS, semi-quantitative RT-PCR expression analysis	758431	
3.5.99.7	gene acdS, sequence comparisons and phylogenetic analysis	757505	
3.5.99.7	gene PST_1815 or acdS, DNA and amino acid sequence determination and analysis, sequence comparisons, quntitative RT-PCR expression analysis, transcriptional organization of the three consecutive genes, PST1814-16, in the genome of Pseudomonas stutzeri strain A1501, overview	757414	
3.5.99.7	identification of a specific ACC deaminase domain region (ACCD-DR) that, when PCR amplified from the soil, produces a variant pool that can be swapped into functional plasmids carrying ACC deaminase-encoding genes. The ACC deaminase domain regions are recombinantly expressed in Escherichia coli. Functional clones of ACC deaminase are selected for in a competition assay based on their capacity to provide nitrogen to Escherichia coli in vitro, DNA and amino acid sequence determination and analysis	755841	
3.5.99.7	Pseudomonas putida UW4 ACC deaminase is cloned into the pET30a (+) vector at the EcoRV/HindIII sites. All single and double mutants are constructed using a Phusion Site Directed Mutagenesis Kit.	694780	
3.5.99.7	subcloned into pET-11d and expressed in Escherichia coli	648260	
3.5.99.7	Using non-transformed canola (Brassica napus) or canola transformed with the ACC deaminase gene from Pseudomonas putida UW4. The transformed canola line has two copies of the ACC deaminase gene under the control of the rootspecific Agrobacterium rhizogenes promoter. This homozygous line is created through Agrobacterium tumefaciens transformation of canola callus culture.	691950	
3.5.99.7	wild-type enzyme and mutants Y268F and Y294F as His6-tagged proteins in Escherichia coli	718894