3.5.5.5 - 670163 3.5.5.5 DNA and amino acid sequence determination and analysis, sequence comparison, cloning and expression as His-tagged protein in Escherichia coli strains JM109 and BL21(DE3) 719629 3.5.5.5 expressed in Escherichia coli BL21(DE3) cells 733558 3.5.5.5 expressed in Escherichia coli JM109 cells 710930, 733193, 734496 3.5.5.5 expressed in Escherichia coli Rosetta2 (DE3) cells 687882 3.5.5.5 expressed in Escherichia coli strain JM109 689852 3.5.5.5 expressed in Pichia pastoris 684655 3.5.5.5 expression in Escherichia coli 755880 3.5.5.5 expression in Escherichia coli BL21 (DE3) 755936 3.5.5.5 expression in Escherichia coli JM 109, simultanuously with cassava (Manihot esculenta) oxynitrilase 717051 3.5.5.5 expression in Escherichia coli strain BL21-Gold (DE3) 718670 3.5.5.5 expression ion Escherichia coli 757638 3.5.5.5 expression under the control of a rhamnose-inducible promoter in Escherichia coli strain JM109 718648 3.5.5.5 overexpression in Escherichia coli BL21 (DE3) and formation ofd inclusion bodies that are active toward mandelonitrile and stable across a broad range of temperature and pH 756265 3.5.5.5 recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease 719297 3.5.5.5 The nitrilase gene from Pseudomonas putida is cloned in plasmid pET 21b(+) and over-expressed as histidine-tagged protein in Escherichia coli strain BL21 (DE3) 694107