3.4.22.54 - 649012, 649026, 649035, 664132 3.4.22.54 cloning of human full-length CDSs in BD Creator(TM) system donor vector 395498 3.4.22.54 COS-7 cells are transfected with C3 construct ssubcloned into pcDNA 3.1 and/or a C-terminal human FLNC construct subcloned into pCMV Tag2B 649029 3.4.22.54 expressed in NIH-3T3 fibroblasts 717589 3.4.22.54 expression in COS-7 cells 680587, 680771 3.4.22.54 expression in Eschericia coli 698178 3.4.22.54 expression of domain IV in Escherichia coli BL21 (DE3) 663960 3.4.22.54 isoforms 643974 3.4.22.54 skeletal muscle enzyme 395498 3.4.22.54 the C-terminally truncated form of the enzyme that comprises the protease cor, domains I and II, alone with its insertion sequence, IS1, and N-terminal leader sequence, NS is expressed in Escherichia coli BL21(DE3) 649031 3.4.22.54 the His6-tagged PEF domain of calpain 3 coexpressed with the PEF domain of the small subunit that has been tagged with an antifreeze protein is expressed in Escherichia coli BL21(DE3) cells 708490