3.4.22.2 - 95668 3.4.22.2 DNA sequence determination and analysis of the proenzyme, expression of the isolated papain prosegment in Escherichia coli strain BL21(DE3) in the insoluble fraction 667324 3.4.22.2 expressed in Escherichia coli 733209 3.4.22.2 gene CpXCP5, genotyping using the peptidase_C1 domain, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis 753097 3.4.22.2 the DNA coding for propapain is cloned and expressed as inclusion bodies at a high level in Escherichia coli BL21(DE3) transformed with two T7 promoter based pET expression vectors, pET30 Ek/LIC and pET28a+, each containing the propapain gene, recombinant propapain is expressed as an insoluble His-tagged fusion protein 700680 3.4.22.2 wild-type papain and D158N variant produced in a baculovirus insect cell expression system 95699