3.4.21.B12 - 647332 3.4.21.B12 adenovirus-mediated ectopic expression of KLK4 in PC-3 cells and DU145 cells 685946 3.4.21.B12 construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3) 647338 3.4.21.B12 DNA and amino acid sequence determination and analysis 647333 3.4.21.B12 DNA sequence determination and analysis, chromosome mapping and genomic organization 647334, 647335, 647336 3.4.21.B12 DNA sequence determination and analysis, chromosome mapping and genomic organization, promotor analysis 647337 3.4.21.B12 expressed in Escherichia coli 681390 3.4.21.B12 expressed in Escherichia coli M15(pREP4) cells and in insect cells (nur hk5) 680680 3.4.21.B12 expressed in Rattus norvegicus and Mus musculus 680174 3.4.21.B12 expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide 647339 3.4.21.B12 expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus 647339 3.4.21.B12 expression of inactive zymogen in human 293 kidney cells 647339 3.4.21.B12 gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9 731763 3.4.21.B12 into the BamH/HindIII restriction sites of the pQE30 vector and expressed in Escherichia coli M15[pREP4] cells 681390 3.4.21.B12 KLK4, 5, 6, and 7 full-length cDNA cloned into vector pRc/RSV. OV-MZ-6 ovarian cancer cells stably co-transfected with plasmids expressing K4-7. These OVKLK4+5+6+7 cells inoculated into the peritoneal cavity of nude mice 664273 3.4.21.B12 KLK4-pIB/V5-His construct expressed in Sf9 cells. PC-3 cell stimulated with KLK4 687699 3.4.21.B12 overexpression of KLK4 in PC-3 cells 689848